The mechanism of the enhanced antioxidant effects

against superoxide anion radicals of reduced water

produced by electrolysis.

Biophys Chem.2004

Jan 1;107(1):71-82.

Hanaoka K, Sun D, Lawrence R, Kamitani Y, Fernandes G.

Bio-REDOX Laboratory Inc.1187-4, Oaza-Ueda-shi, Nagano-ken 386-0001, Japan. hanak@rapid.ocn.ne.jp

We reported that reduced water produced by electrolysis enhanced the antioxidant effects of portion donors such as ascorbic acid (AsA)in a previous paper.

We also demonstrated that reduced water produced by electrolysis of 2 mM NaCI solutions did not show antioxidant effects by itself. We reasoned that the enhancement of antioxidant effects may be due to the increase of the ionic product of water as solvent. The ionic product of water(pKw)was estimated by measurements of pH and by a neutralization titration method. As an indicator of oxidative damage, Reactive Oxygen Species-(ROS) mediated DNA strand breaks were measured by the conversion of supercoiled phiX-174 RFI double-strand DNA to open and linear forms reduced water had a tendency to suppress single-strand breakage of DNA induced by reactive oxygen species produced by H2O2/Cu (II) and HQ/Cu (II) systems. The enhancement of superoxide anion radical dismutation activity can be explained by changes in the ionic product of water in the reduced water.

PMID: 14871602 [PubMed – in process]